HUMAN CYTOMEGALOVIRUS IMMEDIATE-EARLY AND LATE TRANSCRIPTS ARE EXPRESSED IN ALL MAJOR LEUKOCYTE POPULATIONS IN-VIVO

Leukocyte populations were purified by fluorescence-activated cell sor ter and analyzed for human cytomegalovirus (HCMV) DNA and mRNA using p olymerase chain reaction (PCR) and cDNA PCR, respectively, Twenty-two blood samples from 17 patients with active HCMV infection were studied . HCMV DNA was detected most frequently in granulocytes and monocytes but was also found in CD4(+), CD8(+), and CD19(+) lymphocytes. Viral i mmediate early and late mRNA was found in ficoll-enriched mononuclear cells and polymorphonuclear leukocytes. In 11 samples from 9 patients with active HCMV infection, immediate early mRNA was detected in 8 lym phocyte, 6 monocyte, and 8 granulocyte fractions. Late mRNA was detect ed in 7 lymphocyte, 7 monocyte, and 9 granulocyte fractions. The prese nce of viral late mRNA provides strong evidence for infection of these leukocyte populations with HCMV in vivo.