DETECTION OF CHLAMYDIA-PNEUMONIAE TWAR IN HUMAN CORONARY ATHERECTOMY TISSUES

Citation
La. Campbell et al., DETECTION OF CHLAMYDIA-PNEUMONIAE TWAR IN HUMAN CORONARY ATHERECTOMY TISSUES, The Journal of infectious diseases, 172(2), 1995, pp. 585-588
Citations number
15
Categorie Soggetti
Infectious Diseases
ISSN journal
00221899
Volume
172
Issue
2
Year of publication
1995
Pages
585 - 588
Database
ISI
SICI code
0022-1899(1995)172:2<585:DOCTIH>2.0.ZU;2-B
Abstract
Chlamydia pneumoniae TWAR has been associated with coronary heart dise ase by seroepidemiologic studies and direct detection of the organism in atheromatous lesions of coronary arteries and aorta. In this study, 38 fresh tissue specimens from patients with coronary artery lesions that were treated by directional coronary atherectomy were tested for C. pneumoniae, Twenty-three specimens were from patients with primary lesions and 15 were from patients with restenoses. C. pneumoniae was d etected by polymerase chain reaction (PCR), immunocytochemical stain ( ICC), or both in 20 of 38 specimens. Using cell identity markers, the organism was localized to macrophages. Ultrastructural evidence of the organism was found in the 2 specimens examined by transmission electr on microscopy, which were also positive by both ICC and PCR, C. pneumo niae was found more frequently in tissues from restenoses than in prim ary lesions (P = .17). There was no relation between the frequency of detection of the organism and C. pneumoniae-specific antibody titers.