This is a preliminary investigation of a method for the timely monitor
ing of wastewater biochemical oxygen demand (BOD). Many ecological and
economic pressures support the use of BOD measurement methods fast en
ough to prevent process upsets. Since the standard laboratory procedur
e takes five days, and previously used fast tests are unsatisfactory f
or various reasons, tests were made on the Nissin Electric BOD-2000 in
strument, which uses a yeast-based biosensor to measure soluble BOD in
30 min. It has been used successfully in the pharmaceutical and food
industries. An initial attempt was made to place the instrument in fie
ld service. This attempt was unsuccessful, so the present study concen
trated on comparing its operation in the laboratory with the results o
f the standard five-day BOD test (BOD,) procedure. The two types of te
sts were compared for samples from Terminal Island Treatment Plant (TI
TP), Bureau of Sanitation of the city of Los Angeles, using various co
mbinations of filter porosities and wastewater sources in an attempt t
o establish a measurement routine that would not suffer from clogging
problems that plagued the held test. Under these conditions the result
s from the instrument are excellent, and we briefly discuss further wo
rk needed to bring it into field use. This test is believed to be the
first effort to assess the capability of this technology in a wastewat
er application in the United States.