F. Borguet et al., STUDY OF THE CHROMIUM BINDING IN PLASMA OF PATIENTS ON CONTINUOUS AMBULATORY PERITONEAL-DIALYSIS, Clinica chimica acta, 238(1), 1995, pp. 71-84
The distribution of chromium (Cr) among the proteins in plasma of thre
e continuous ambulatory peritoneal dialysis (CAPD) patients and in dia
lysis solutions was studied with 'in vitro' and 'in vivo' Cr-51-labell
ed plasma. Radiolabelling allows the kinetics of newly added Cr to be
investigated. The separation of the proteins was achieved by ion excha
nge fast protein liquid chromatography, ensuring quantitative recoveri
es of the Cr-51 activity, transferrin and albumin. In all fractions th
e proteins were qualitatively characterised by iso electric focusing.
The concentrations of transferrin and albumin were determined by nephe
lometry. The Cr-51 distribution among the plasma proteins was similar
for the three patients. The Cr-51 activity was mainly bound to transfe
rrin and, in the beginning of the incubation, in a lesser degree also
to albumin. After 1-6 h a shift was observed of the Cr-51 from albumin
to an unidentified low molar mass complex (+/- 5000 Da). Two Cr-51 sp
ecies showed up in the subsequently drained dialysate, but they could
not be identified.