Pe. Rollin et al., SHORT REPORT - ISOLATION AND PARTIAL CHARACTERIZATION OF A PUUMALA-VIRUS FROM A HUMAN CASE OF NEPHROPATHIA-EPIDEMICA IN FRANCE, The American journal of tropical medicine and hygiene, 52(6), 1995, pp. 577-578
Citations number
10
Categorie Soggetti
Public, Environmental & Occupation Heath","Tropical Medicine
Puumala (PUU) virus (Bunyaviridae: Hantavirus), the etiologic agent of
nephropathia epidemica (NE), the mild form of hemorrhagic fever with
renal syndrome, is enzootic in Europe and has been known to occur in F
rance since 1983. We report the first isolation of PUU virus in France
and western Europe from a case of NE acquired in France. The virus wa
s isolated from a serum collected in the acute phase of the clinical c
ourse by successive blind passages in Vero E6 cells. Serologic typing
using monoclonal antibodies confirmed the identity of the virus as PUU
. The sequence of an 832-nucleotide fragment of the virus medium RNA s
egment obtained by the polymerase chain reaction (PCR) also classified
it as a PUU virus. The sequence of this isolate from a human case in
France is closely related to the sequence of a PUU virus obtained by t
he PCR from a German patient.