Wz. Tang et al., PERSISTENCE IN SOIL OF THE PLANT-GROWTH PROMOTING RHIZOBACTERIUM PSEUDOMONAS-PUTIDA GR12-2 AND GENETICALLY MANIPULATED DERIVED STRAINS, Canadian journal of microbiology, 41(6), 1995, pp. 445-451
Transformation of the plant growth promoting rhizobacterium Pseudomona
s putida GR12-2 with broad-host-range vectors can affect the growth of
the bacterium, its ability to promote root elongation of canola seedl
ings under gnotobiotic conditions, and its persistence in soil. Plasmi
d transformants, and a transposon-mutagenized derivative of P. putida
GR 12-2, fell into two classes with respect to these three attributes:
strains that were clearly diminished in these capabilities and strain
s that behaved like the nontransformed wild type. These differences ca
n be accounted for by the imposition of a metabolic load that is creat
ed by some types of genetic modification that results in a physiologic
al impairment of the modified bacterium and decreases its ability to f
unction as a plant growth promoting rhizobacterium.