N. Milanovich et al., WHITE-LIGHT HOLE-BURNING OF TO-PRO-3 BOUND TO OLIGOMERIC DNA, Molecular crystals and liquid crystals science and technology. Section A, Molecular crystals and liquid crystals, 291, 1996, pp. 147-154
Binding of the dye TO-PRO-3 iodide to alternating GC and AT oligomers
of DNA is investigated by absorption and hole burning spectroscopies.
This dye is one of a series extensively used for nucleic acid staining
. In addition to narrow laser induced holes, the dye/oligomer complexe
s also exhibit white light induced hole burning. These holes are indic
ative of a dye/DNA binding mode in addition to the previously identifi
ed externally bound and intercalative modes. Possible structures of th
is mode are considered.