CONDENSATION OF GLUCOSE BY THE REVERSED HYDROLYSIS REACTION OF GLUCOAMYLASE .3. EFFECT OF ORGANIC-SOLVENTS AND IMMOBILIZATION FORMS

Enzymatic synthesis of oligosaccharides by glucoamylase in the presenc e of organic solvents and type of support material used for immobiliza tion were investigated. Using an organic solvent caused an increase in the yield of oligosaccharides by reducing the water content and it ap pears that the yield of oligosaccharides in water-miscible solvent is smaller than in water-immiscible solvent. The data obtained clearly de monstrated that the type of carrier used for enzyme immobilization has influence mainly on isomaltose synthesis. The higher yield of oligosa ccharides (especially isomaltose) was attained when using immobilized glucoamylase preparation resulted from pore diffusion and tortuosity e ffects.