SGFI, A NEW TYPE-II RESTRICTION-ENDONUCLEASE THAT RECOGNIZES THE OCTANUCLEOTIDE SEQUENCE 5'-GCGAT CGC-3'/

Authors
KAPPELMAN JR BRADY M KNOCHE K MURRAY E SCHOENFELD T WILLIAMS R VESSELINOVA N
Citation
Jr. Kappelman et al., SGFI, A NEW TYPE-II RESTRICTION-ENDONUCLEASE THAT RECOGNIZES THE OCTANUCLEOTIDE SEQUENCE 5'-GCGAT CGC-3'/, Gene, 160(1), 1995, pp. 55-58
Citations number
13
Categorie Soggetti
Genetics & Heredity
Journal title
Gene → ACNP
ISSN journal
03781119
Volume
160
Issue
1
Year of publication
1995
Pages
55 - 58
Database
ISI
SICI code
0378-1119(1995)160:1<55:SANTRT>2.0.ZU;2-9
Abstract
A new restriction endonuclease (ENase), Sgfl, has been isolated from t he bacterium Streptomyces sp. Sgfl recognizes the 8-bp palindrome 5'-G CGATCGC-3' and cleaves double-stranded DNA after the T in this sequenc e, producing a two-base 3' overhang compatible with PvuI termini. SgfI is a rare-cutting ENase and should be useful for megabase mapping exp eriments.