SEQUENCE AND STRUCTURE OF PENICILLIUM-CHRYSOGENUM PHOG, HOMOLOGOUS TOAN ACID PHOSPHATASE-ENCODING GENE OF ASPERGILLUS-NIDULANS

A Penicillium chrysogelzum (Pc) gene (phoG), homologous to an Aspergil lus nidulans (An) gene which confers phosphate non-repressible acid ph osphatase (APase) activity, has been cloned and sequenced. The 2.9-kb genomic sequence corresponds to two ORFs of 149 and 1630 bp encoding a protein of 593 amino acids (aa). As verified by cDNA sequencing, the coding region is interrupted by an 85-bp intron. The deduced aa sequen ce of phoG reveals 61% aa identity to the translated long ORF of the A n APase-encoding gene. Northern blot analysis indicated a 2.3-kb trans cript in approximately equivalent amount in mycelia grown under differ ent phosphate concentrations.