GRANULOPOIESIS INHIBITING FACTOR - SYNTHE SIS AND BIOLOGICAL-ACTIVITY

Three schemes of synthesis for pentapeptide Glp-Glu-Asp-Cys-Lys-OH wer e compared was carried out. Acetamidomethyl protection was used for th e mercapto group of cysteine, For the same purpose, cystine was used a s the starting compound for synthesis. The optimal method was shown to be the solid phase method with S-acetamidomethyl cysteine protection that can be removed by mercuric acetate before the cleavage of a pepti de from a polymer. The stabilized peptide inhibits proliferation of bo ne marrow cells of patients with chronic myeloleukemia 5- to 20-fold a nd has a less pronounced effect (up to 2-fold inhibition) on periphera l blood cells. Thus, its application for the therapy of hemoblastoses is promising.