CLONING CDNA-ENCODING F-V-FRAGMENTS OF TH E LIGHT AND HEAVY-CHAINS OFTHE MONOCLONAL-ANTIBODY AGAINST HUMAN INTERLEUKIN-2

DNA fragments coding for the variable fragments of the heavy and light chains of the immunoglobulin G(1) against human recombinant interleuk in-2 were produced using reverse transcription of the total RNA isolat ed from the murine hybrid myeloma cell line LNKB-2 followed by amplifi cation of the RNA-DNA duplexes with degenerated primers. The fragments obtained were cloned into the plasmid pGEM7-Zf(+) and their structure s were determined. The fragments cloned were proved to encode the F-v- fragments by sequencing N-termini of the light and heavy chains of the antibody.