Ap. Dessai et al., PLANT-REGENERATION OF SWEET-POTATO (IPOMOEA-BATATAS L) FROM LEAF EXPLANTS IN-VITRO USING A 2-STAGE PROTOCOL, Scientia horticulturae, 62(4), 1995, pp. 217-224
Leaf explants of sweetpotato (Ipomoea baratas L. (Lam.)) were cultured
on Murashige and Skoog medium (MS) with varying levels (0.2-1 mg l(-1
)) of 2,4-dichlorophenoxyacetic acid (2,4-D; stage I) and transferred
to a medium with zeatin riboside (0.2-0.4 mg l(-1); stage II). The hig
hest frequency (over 80%) shoot regeneration occurred in the genotype
PI 318846-3 cultured on MS + 2,4-D (0.2 mg l(-1)) for 3 days and then
transferred to MS + zeatin riboside (0.2 mg l(-1)). Addition of zeatin
riboside in the first medium reduced the frequency of shoot regenerat
ion. Of the 27 genotypes, 19 exhibited high frequency shoot regenerati
on, and eight were completely recalcitrant. The developmental stage of
the leaf was critical, as young leaves from the apical portions of th
e stem were the most regenerative. Regenerated shoots rooted readily a
nd the plants transferred to soil in the greenhouse appeared normal.