Bjm. Zonneveld et Al. Vanderzanden, THE RED ADE MUTANTS OF KLUYVEROMYCES-LACTIS AND THEIR CLASSIFICATION BY COMPLEMENTATION WITH CLONED ADE1 OR ADE2 GENES FROM SACCHAROMYCES-CEREVISIAE, Yeast, 11(9), 1995, pp. 823-827
Seventy-six red adenine mutants of Kluyveromyces lactis were isolated.
By complementation they could be assigned to two groups with 31 and 4
5 mutants. Transformation of several strains from each group with plas
mids containing the Saccharomyces cerevisiae ADE1 or ADE2 gene showed
that the largest group was ade2 and the other group was ade1. Several
previously isolated 'ade1' mutants were classified to either group and
given new gene and allele numbers. ADE1 was localized at chromosome I
II, closely linked to the mating type gene, making it a convenient mar
ker for mating type. ADE2 was localized at chromosome V.