EFFICIENCY OF THE MICROBANK-SYSTEM FOR TH E CONSERVATION OF MICROORGANISMS IMPORTANT IN VETERINARY-MEDICINE AND OTHER MICROORGANISMS WHICH COULD NOT EASILY BE CULTIVATED

Citation
Ke. Seidel et M. Gareis, EFFICIENCY OF THE MICROBANK-SYSTEM FOR TH E CONSERVATION OF MICROORGANISMS IMPORTANT IN VETERINARY-MEDICINE AND OTHER MICROORGANISMS WHICH COULD NOT EASILY BE CULTIVATED, Berliner und Munchener Tierarztliche Wochenschrift, 108(6), 1995, pp. 215-220
Citations number
14
Categorie Soggetti
Veterinary Sciences
ISSN journal
00059366
Volume
108
Issue
6
Year of publication
1995
Pages
215 - 220
Database
ISI
SICI code
0005-9366(1995)108:6<215:EOTMFT>2.0.ZU;2-1
Abstract
In this paper the conservation of different bacteria, yeasts and molds from diagnostic material was examined with a commercial deep freeze s ystem. 137 bacteria isolated from the stomachs of dogs, cats and pigs and from duodenal juice of cats and 7 isolates of yeasts and fungi fro m diagnostic material were conservated with the deep freeze system MIC ROBANK-TM (Mast Diagnostica). Furthermore 62 Helicobacter pylori-isola tes and 1 Helicobacter felis-isolate were conservated with this system . After a storage period of 24 hours up to 20 months the isolates were recultured. 96% of the conservated microorganisms could be grown. It was not possible to cultivate 1 fungus-isolate (Fusarium sp.). 2 Helic obacter pylori-isolates, spiral bacteria from the stomach of a pig and a Clostridium tyrobutyricum-isolate were only recultured after 24 hou rs, 1 isolate Moraxella sp. after 2 months and another 4 anaerobe isol ates after 5 months. The system MICROBANK-TM proved to be suitable for conservation and was also efficient for the conservation of microorga nisms, which could not easily be cultivated.