EFFICIENCY OF THE MICROBANK-SYSTEM FOR TH E CONSERVATION OF MICROORGANISMS IMPORTANT IN VETERINARY-MEDICINE AND OTHER MICROORGANISMS WHICH COULD NOT EASILY BE CULTIVATED
Ke. Seidel et M. Gareis, EFFICIENCY OF THE MICROBANK-SYSTEM FOR TH E CONSERVATION OF MICROORGANISMS IMPORTANT IN VETERINARY-MEDICINE AND OTHER MICROORGANISMS WHICH COULD NOT EASILY BE CULTIVATED, Berliner und Munchener Tierarztliche Wochenschrift, 108(6), 1995, pp. 215-220
In this paper the conservation of different bacteria, yeasts and molds
from diagnostic material was examined with a commercial deep freeze s
ystem. 137 bacteria isolated from the stomachs of dogs, cats and pigs
and from duodenal juice of cats and 7 isolates of yeasts and fungi fro
m diagnostic material were conservated with the deep freeze system MIC
ROBANK-TM (Mast Diagnostica). Furthermore 62 Helicobacter pylori-isola
tes and 1 Helicobacter felis-isolate were conservated with this system
. After a storage period of 24 hours up to 20 months the isolates were
recultured. 96% of the conservated microorganisms could be grown. It
was not possible to cultivate 1 fungus-isolate (Fusarium sp.). 2 Helic
obacter pylori-isolates, spiral bacteria from the stomach of a pig and
a Clostridium tyrobutyricum-isolate were only recultured after 24 hou
rs, 1 isolate Moraxella sp. after 2 months and another 4 anaerobe isol
ates after 5 months. The system MICROBANK-TM proved to be suitable for
conservation and was also efficient for the conservation of microorga
nisms, which could not easily be cultivated.