THE FREQUENCY OF 4977-BASE-PAIR DELETION OF MITOCHONDRIAL-DNA IN VARIOUS TYPES OF LIVER-DISEASE AND IN NORMAL LIVER

Citation
S. Fukushima et al., THE FREQUENCY OF 4977-BASE-PAIR DELETION OF MITOCHONDRIAL-DNA IN VARIOUS TYPES OF LIVER-DISEASE AND IN NORMAL LIVER, Hepatology, 21(6), 1995, pp. 1547-1551
Citations number
27
Categorie Soggetti
Gastroenterology & Hepatology
Journal title
ISSN journal
02709139
Volume
21
Issue
6
Year of publication
1995
Pages
1547 - 1551
Database
ISI
SICI code
0270-9139(1995)21:6<1547:TFO4DO>2.0.ZU;2-5
Abstract
Using a polymerase chain reaction (PCR) method, we tested for the hepa tic mitochondrial DNA (mtDNA) deletion in 40 hepatic tumors (28 hepato cellular carcinomas [HCCs], 9 other malignant tumors, and 3 benign tum ors) and in the livers of 71 patients, including 16 pediatric patients with end-stage liver disease who underwent living related donor Liver transplantation and 16 liver donors. A 4977 base pair (bp) deletion o f mtDNA was detected in 36 of 55 specimens of non-tumor portions of ad ult liver (65.5%), However, none of the specimens obtained hom cirrhot ic livers of the 16 pediatric patients younger than 13 years of age ha d the 4977 bp deletion. The frequency of mtDNA deletion was significan tly decreased compared with normal liver in HCCs (7 of 28) and other m alignant Liver tumors (2 of 9). The frequency of this deletion was unr elated to the presence of liver cirrhosis, patient's gender, hepatitis B virus surface antigen status, and hepatitis C virus antibody status .