SIMULTANEOUS VISUALIZATION OF Q-BANDS AND FISH SIGNALS USING A NOVEL FLUOROCHROME

Authors
KONDOH Y ONO T KAGIYAMA N FUJITA S MOMIYAMA M HORI SH YOSHIDA MC
Citation
Y. Kondoh et al., SIMULTANEOUS VISUALIZATION OF Q-BANDS AND FISH SIGNALS USING A NOVEL FLUOROCHROME, Cytogenetics and cell genetics, 71(1), 1995, pp. 96-98
Citations number
10
Categorie Soggetti
Cell Biology","Genetics & Heredity
Journal title
Cytogenetics and cell genetics → ACNP
ISSN journal
03010171
Volume
71
Issue
1
Year of publication
1995
Pages
96 - 98
Database
ISI
SICI code
0301-0171(1995)71:1<96:SVOQAF>2.0.ZU;2-B
Abstract
We developed a new HNPP-azo dye method for detection of fluorescence i n situ hybridization (FISH) signals on Q-banded chromosomes by use of a newly synthesized fluorochrome, HNPP (3-hydroxy-N-2'-biphenyl-2-naph thalenecarboxamide phosphate ester), which reacts enzymatically with a lkaline phosphatase and azo dye. The FISH staining method permits simu ltaneous detection of orange HNPP signals on chromosomal sites labeled by Q-banding, allowing the assignment of small (440-1,200 bp) probes.