J. Dassarma et al., ANTIBODY TO FOLIC-ACID - INCREASED SPECIFICITY AND SENSITIVITY IN ELISA BY USING EPSILON-AMINOCAPROIC ACID MODIFIED BSA AS THE CARRIER PROTEIN, Journal of immunological methods, 184(1), 1995, pp. 1-6
For raising high titre and specific antibody to haptens or drugs, epsi
lon-aminocaproic acid modified bovine serum albumin (epsilon-ACA-BSA)
was prepared for use as a carrier protein. Folic acid (FA) was coupled
to epsilon-ACA-BSA, Imj.BSA and BSA for raising antibodies in rabbits
. Enhancement of FA immunogenicity with FA-ACA-BSA was observed. Apart
from determination of titre by indirect ELISA, dose-response behaviou
r and specificity of these antisera were also compared. FA-ACA-BSA ant
ibody showed high sensitivity and specificity. Using this antibody, an
ELISA method for the determination of FA was developed. The study pro
vides a simple approach to raise highly specific and high titre antibo
dy against small molecules.