CYTOGENETIC STUDY OF BOVINE OOCYTES MATURED IN-VITRO

Described in the present paper is a cytogenetic study of bovine oocyte s matured in vitro. The cumulus-oocyte complexes (COG), punctured from ovaries recovered in a local slaughterhouse, were classified into 3 g roups according to follicular diameter: 1 to 4mm, 5 to 8mm and 9 to 13 mm. Metaphases available for observation were classified as metaphase I, haploid and diploid metaphase II. High levels of haploid metaphase s II (90.6, 86.9 and 94.4 %) among the 3 groups of follicular sizes in dicated successful meiotic resumption during in vitro maturation and s uggested that cytoplasmic maturation may be responsible for low develo pmental rate after IVM, IVF and in vitro development (IVD).