GENE-BASED SEQUENCE-TAGGED-SITES (STSS) AS THE BASIS FOR A HUMAN GENEMAP

Using our data set of 3,143 single pass sequences from human brain cDN A libraries, we have developed a strategy in which gene-based sequence -tagged-sites (STSs), derived from 3'untranslated regions of human cDN As, are rapidly assigned to megabase-insert yeast artificial chromosom es and somatic cell hybrids to generate regional gene mapping data, Em ploying this approach, we have mapped 318 cDNAs, representing 308 huma n genes. Ninety-two of these mapped to regions implicated in human gen etic diseases, identifying them as candidate genes. Extension of this strategy has the potential to result in virtually every human gene hav ing, at its 3'end, its own associated STS, with each STS in turn speci fying both a corresponding genomic clone and a specific regional locat ion in the genome.