MECHANISM OF ACTIVATION OF HUMAN NEUTROPHIL GELATINASE-B - DISCRIMINATING BETWEEN THE ROLE OF CA2+ IN ACTIVATION AND CATALYSIS

Gelatinase B is a Zn2+- and Ca2+ dependent endopeptidase that is secre ted from cells as an inactive proenzyme. The enzyme can be activated i n vitro by organomercurial compounds and by trypsin. The role of Ca2in autoproteolytic processing initiated by 4-aminophenylmercuric aceta te and trypsin and in catalytic activity of the activated enzyme was i nvestigated by zymography and by kinetic analysis. Treatment of unglyc osylated 57.5-kDa pro-gelatinase B with 4-aminophenylmercuric acetate (1 mM) in the absence of Ca2+ generated a 49-kDa inactive intermediate (E'), whereas a 41.5-kDa active species (E '') was generated in the p resence of Ca2+ (5 mM). Upon addition of Ca2+ to the reaction mixture of Ca2+ depleted E' or E '' at 37 degrees C, E' showed a lag period in generation of the product as a function of time, but E '' presented a n immediate activity. The appearance of enzymatic activity of E' corre lated with the generation of the E '' species. NH2-terminal sequence a nalyses showed that E' and E '' had the same NH2 termini. i.e. Met-75, suggesting that Ca2+-dependent removal of COOH terminus of E' is requ ired for activation of the enzyme, Treatment of pro-gelatinase B with trypsin in the absence of Ca2+, led to degradation of the enzyme, In t he presence of Ca2+, trypsin processed the pro enzyme to a 40-kDa acti ve species. In contrast to E '', this active species did not require C a2+ for activity. The Ca2+ dependence of E '' activity was also abolis hed by treatment of the enzyme with trypsin. NH2-terminal sequence ana lysis indicated that amino acid residues 75-87 had been re moved from the NH2 terminus of E '' by trypsin, suggesting that these residues ar e responsible for the Ca2+-dependent activity of the enzyme. Removal o f Ca2+ and catalytic Zn2+ inhibited the activities of both E '' and tr ypsin treated E ''. In the absence of Ca2+, either Zn2+, Co2+, Mn2+, o r Cd2+ was able to restore the activity of trypsin-treated E ''. None of the divalent cations tested however, was able to stimulate the acti vity of E '' in the absence of Ca2+. These experiments further suggest that binding of Ca2+ to E '' or removal of the NH2-terminal residues of the enzyme by trypsin induces a conformational change in the protei n and makes the active site of the enzyme accessible to various metal ions rendering the enzyme active.