CD AND NMR DETERMINATION OF THE SOLUTION STRUCTURE OF A PEPTIDE CORRESPONDING TO T4 LYSOZYME RESIDUES-38-51

Solid phase methods have been used to synthesise a peptide correspondi ng to residues 38-51 of T4 lysozyme. The peptide, LYS(38-51), encompas ses helix B in the crystal structure of T4 lysozyme. CD and H-1-NMR an alysis showed that the peptide was unstructured in aqueous solution bu t adopted a helical conformation in the more hydrophobic environment p rovided by 50% TFE and SDS micelles. The solution structure derived fr om the NMR data was similar to that of the helix in the X-ray structur e, although there was some fraying at the N-terminus.