JUN-B ONCOGENE ABERRATIONS IN CERVICAL-CANCER CELL-LINES

We describe here structural and expression analysis of the jun-B oncog ene in two cervical cancer cell lines. In the CC7T-a cell line, result s from both Southern analysis and cDNA cloning studies revealed the ex istence of two structurally altered jun-B alleles besides the normal g ene. One of the altered alleles was due to a type 16 human papillomavi rus (HPV-16) integration event, whereas the other allele was a consequ ence of a chromosomal translocation involving chromosome 19 (jun-B) an d an EST182 locus residing in chromosome 15. In the HeLa cell line, wh ich contains integrated HPV-18, an apparent structural aberration, a 3 -fold amplification and a 3-fold overexpression of the jun-B gene were observed, Our observations suggest that deregulation of the jun-B gen e expression may have contributed to the transformation process in the se two cervical cancer cell lines.