CONFIRMATION OF THE IDENTITY OF HUMAN SKELETAL REMAINS USING MULTIPLEX PCR AMPLIFICATION AND TYPING KITS

Citation
Mn. Hochmeister et al., CONFIRMATION OF THE IDENTITY OF HUMAN SKELETAL REMAINS USING MULTIPLEX PCR AMPLIFICATION AND TYPING KITS, Journal of forensic sciences, 40(4), 1995, pp. 701-705
Citations number
32
Categorie Soggetti
Medicine, Legal
ISSN journal
00221198
Volume
40
Issue
4
Year of publication
1995
Pages
701 - 705
Database
ISI
SICI code
0022-1198(1995)40:4<701:COTIOH>2.0.ZU;2-X
Abstract
The identity of human skeletal remains found in a wooded area approxim ately one year after the person was reported missing was provisionally established by routine methods and circumstantial evidence. Multiplex PCR systems-the AmpfiType(R) PM PCR Amplification and Typing Kit and the GenePrint(TM) STR Tripler Amplification and Typing Kit-were used t o confirm the identification. DNA profiles from femur bone from the re mains were compared with profiles derived from head hairs from a hair- brush recovered in the missing woman's apartment. In addition, a sex t yping procedure using the X-Y homologous gene amelogenin was carried o ut. This is the first report of a case using commercially available mu ltiplex PCR amplification and typing kits to confirm the identity of s keletal remains.