METROLOGICAL OPPORTUNITIES OF THE DYNAMIC-MODE OF OPERATING AN ENZYMEAMPEROMETRIC BIOSENSOR

An algorithm for generating calibration curves that reflects the pecul iarities of the dynamic mode of operating of the amperometric biosenso r, created on the base of numerical simulations of the sensor behaviou r, is proposed. The most important steps are: selection of the informa tive quantity about the sensor current versus time relationship and th e calibration curve processing. Five different informative quantities are tested as well as two ways of representation of calibration curves . The object of study is enzyme catalyzed selective reaction. The valu es of the kinetic constants used reflect the conversion of glucose to gluconolacton catalyzed by glucose oxidase (GOD) and catalase (CAT). T he results show that sensors with different metrological characteristi cs can be created by means of a selection of informative quantity of t he signal of one biosensor transducer. In addition there appears to be a possibility of facilitating the calibration procedure and prolongin g the life of the biosensor.