Hydrolysis of alkanedicarboxylic acid dimethyl esters using esterase f
rom Pseudomonas putida MR-2068 gave exclusively pure monoesters. Hydro
lytic activities were dependent on the carbon chain length of the subs
trates. This esterase also catalysed enantio- and regio-selective hydr
olysis of alpha-methylalkanedicarboxylic acid dimethyl esters.