Ls. Bonnington et al., P(CH2OH)(3)-POLYETHERAMINE-DERIVED POLYMERIC FILMS FOR ENZYME IMMOBILIZATION, Enzyme and microbial technology, 17(8), 1995, pp. 746-750
Polymer films formed by the condensation of polyetheramines with P(CH2
OH)(3) were synthesized and covalently coupled to the enzyme urease vi
a the intrinsic reactive P-CH2OH hydroxyl groups. Urease activities of
1,140 U g(-1) and 5.5 U cm(-2) of polymer film were achieved without
any additional coupling agents. Upon oxidation of the phosphorus, the
intrinsic hydroxyl groups were inactivated and the resulting urease ac
tivities decreased by over 98%.