P(CH2OH)(3)-POLYETHERAMINE-DERIVED POLYMERIC FILMS FOR ENZYME IMMOBILIZATION

Authors
BONNINGTON LS HENDERSON W PETACH HH
Citation
Ls. Bonnington et al., P(CH2OH)(3)-POLYETHERAMINE-DERIVED POLYMERIC FILMS FOR ENZYME IMMOBILIZATION, Enzyme and microbial technology, 17(8), 1995, pp. 746-750
Citations number
14
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Enzyme and microbial technology → ACNP
ISSN journal
01410229
Volume
17
Issue
8
Year of publication
1995
Pages
746 - 750
Database
ISI
SICI code
0141-0229(1995)17:8<746:PPFFEI>2.0.ZU;2-B
Abstract
Polymer films formed by the condensation of polyetheramines with P(CH2 OH)(3) were synthesized and covalently coupled to the enzyme urease vi a the intrinsic reactive P-CH2OH hydroxyl groups. Urease activities of 1,140 U g(-1) and 5.5 U cm(-2) of polymer film were achieved without any additional coupling agents. Upon oxidation of the phosphorus, the intrinsic hydroxyl groups were inactivated and the resulting urease ac tivities decreased by over 98%.