THE EFFECTS OF INTRACANAL MEDICAMENTS, FILLERS, AND SEALERS ON THE ATTACHMENT OF HUMAN GINGIVAL FIBROBLASTS TO AN EXPOSED DENTIN SURFACE FREE OF A SMEAR LAYER
Lg. Breault et al., THE EFFECTS OF INTRACANAL MEDICAMENTS, FILLERS, AND SEALERS ON THE ATTACHMENT OF HUMAN GINGIVAL FIBROBLASTS TO AN EXPOSED DENTIN SURFACE FREE OF A SMEAR LAYER, Journal of periodontology, 66(7), 1995, pp. 545-551
TO DATE THERE HAS BEEN VERY LITTLE RESEARCH into the possible effects
of endodontic therapy on regeneration of the lost periodontal attachme
nt. The objective of this study was to examine the effects of endodont
ic medicaments on fibroblast attachment to dentin surfaces free of a s
mear layer. Pulp chambers of extracted third molars were filled with o
ne of the following medicaments:gutta-percha with Roth's zinc oxide an
d eugenol-based sealer, warm gutta-percha with sealer, warm gutta-perc
ha without sealer, calcium hydroxide, formocresol, cotton pellet, or l
eft empty. A predetermined dentin surface area was then inoculated wit
h human gingival fibroblasts at a concentration of 2 x 10(4) cells per
mi. The cells were allowed to adhere to the dentin surface for either
4 or 24 hours, then cell attachment was quantified using a methyl-tet
razolium assay, The data were analyzed using a Kruskal-Wallis one-way
analysis of variance and Dunn's multiple comparison test. It was deter
mined that fibroblast attachment was significantly reduced when expose
d to formocresol or warm gutta-percha without sealer at both the 4 and
24 hour interval (P less than or equal to 0.05). This suggests that t
he use of formocresol or warm gutta-percha without sealer in a root ca
nal may impede periodontal wound healing and regeneration.