F. Berditchevski et al., A NOVEL LINK BETWEEN INTEGRINS, TRANSMEMBRANE-4 SUPERFAMILY PROTEINS (CD63 AND CD81), AND PHOSPHATIDYLINOSITOL 4-KINASE, The Journal of biological chemistry, 272(5), 1997, pp. 2595-2598
Enzymatic and immunochemical assays show a phosphatidylinositol 4-kina
se in novel and specific complexes with proteins (CD63 and CD81) of th
e transmembrane 4 superfamily (TM4SF) and an integrin (alpha(3) beta(1
)). The size (55 kDa) and other properties of the phosphatidylinositol
4-kinase (PI 4-K) (stimulated by nonionic detergent, inhibited by ade
nosine, inhibited by monoclonal antibody 4CG5) are consistent with PI
4-K type II. Not only was PI 4-K associated with alpha(3) beta(1)-CD63
complexes in alpha(3)-transfected K562 cells, but also it could be co
-purified from CD63 in untransfected K562 cells lacking alpha(3) beta(
1). Thus, TM4SF proteins may link PI 4-K activity to the alpha(3) beta
(1) integrin. The alpha(5) beta(1) integrin, which does not associate
with TM4SF proteins, was not associated with PI 4-K. Notably, alpha(3)
beta(1)-CD63-CD81-PI 4-K complexes are located in focal complexes at
the cell periphery rather than in focal adhesions. The novel linkage b
etween integrins, transmembrane 4 proteins, and phosphoinositide signa
ling at the cell periphery may play a key role in cell motility and pr
ovides a signaling pathway distinct hom conventional integrin signalin
g through focal adhesion kinase.