G. Bertazza et al., LIGHT EFFECTS ON IN-VITRO ROOTING OF PEAR CULTIVARS OF DIFFERENT RHIZOGENIC ABILITY, Plant cell, tissue and organ culture, 41(2), 1995, pp. 139-143
The effect of varying light regimes on in vitro rooting of microcuttin
gs of two pear (Pyrus communis L.) cultivars was investigated. Culture
s of the easy-to-root 'Conference' and the difficult-to-root 'Doyenne
d'Hiver' were incubated for 21 days with or without indole-3-butyric a
cid (IBA) in the medium in darkness or under continuous far-red (8 mu
mol m(-2) s(-1), blue, white or red (15 or 36 mu mol m(-2) s(-1)) ligh
t. 'Conference' rooted without IBA when exposed to red, blue or white
light while no rooting was observed under far-red light and in darknes
s. The high rooting efficiency under red and, by contrast, the inhibit
ion under far-red light and darkness suggest the involvement of the ph
ytochrome system in rhizogenesis. The addition of IBA to the culture m
edium enhanced root production under all light regimes in both cultiva
rs. Red light, especially at the lower photon fluence rate, had a posi
tive effect by increasing root extension (number X length of roots) an
d stimulating secondary root formation.