MICROPROPAGATION OF MATURE SIBERIAN ELM IN 2 STEPS

A simple and reliable two-step micropropagation system was developed f or mature Siberian elm (Ulmus pumila L.). Shoot-tip cultures were init iated and multiplied on a modified Murashige and Skoog medium suppleme nted with 1 mu M benzyladenine, B5 vitamins, solidified with 0.22% Gel rite, and subcultured weekly. Proliferated shoots 2-3 cm in length roo ted successfully in sterile and non-sterile soil mix at 100% efficienc y. Healthy plants were acclimatized to the ambient environment.