The amperometric method using the flow injection system has been devel
oped for the determination of bilirubin. Bilirubin is oxidized to bili
verdin at the glassy carbon working electrode. The optimum conditions
were investigated. Linear calibration curve was obtained between 1.0x1
0(-6) and 1.0x10(-3)M, with a sampling rate of 20 samples h(-1) and a
relative standard deviation of 3.2%. The limit of detection is 4.0x10(
-7) M. Interference of hemoglobin was not observed.