LOSS OF HETEROZYGOSITY ON CHROMOSOME ARMS 5Q, 11P, 11Q, 13Q, AND 16P IN HUMAN TESTICULAR GERM-CELL TUMORS

Citation
Rma. Aljehani et al., LOSS OF HETEROZYGOSITY ON CHROMOSOME ARMS 5Q, 11P, 11Q, 13Q, AND 16P IN HUMAN TESTICULAR GERM-CELL TUMORS, Genes, chromosomes & cancer, 13(4), 1995, pp. 249-256
Citations number
37
Categorie Soggetti
Oncology,"Genetics & Heredity
Journal title
ISSN journal
10452257
Volume
13
Issue
4
Year of publication
1995
Pages
249 - 256
Database
ISI
SICI code
1045-2257(1995)13:4<249:LOHOCA>2.0.ZU;2-2
Abstract
To identify common regions of deletion in human testicular germ cell t umors (TGCTs), we have screened tumors from 33 patients for loss of he terozygosity (LOH) using Southern blot analysis with 39 polymorphic ma rkers covering 21 chromosome arms. Losses in more than 2 tumors and oc curring at a frequency of >10% were found on chromosome arms 5q, 11p, 11q, 13q, and 16p, the highest being on chromosome arm 5q (19%). It is suggested that tumor suppressor genes on 5q among others may be invol ved in testicular tumorigenesis and that LOH in this region requires f urther investigation. No losses were found on 12q and 17p despite the fact that the most common cytogenetic abnormality in TGCTs is an i(12p ) and that the TP53 gene on 17p is the most frequently mutated gene in human cancers. The level of allelic imbalance varied considerably fro m one chromosome region to another (0-80%) and did not generally refle ct the pattern of LOH. It tended to be high in overrepresented regions of the genome, 1q, 7p, and 12p. The tumor from one patient had a semi nomatous component and a less differentiated component. We provide evi dence for a common origin of both components and show that it is likel y that this tumor has progressed from the seminoma to the less differe ntiated histology. (C) 1995 Wiley-Liss, Inc.