L. Llewellyn et al., CLONING AND CHARACTERIZATION OF A FISH ALDOLASE-B GENE, Biochimica et biophysica acta, N. Gene structure and expression, 1263(1), 1995, pp. 75-78
A full length cDNA clone representing an aldolase mRNA was isolated fr
om a sea bream (Sparus aurata) liver cDNA library. Sequencing of this
clone revealed it to encode a 364 amino acid protein with 74% amino ac
id identity to human aldolase B and slightly lower similarity to human
aldolase A and C. In view of the sequence data and of Northern blot a
nalysis showing strong expression of a 1.6 kb transcript in liver it w
as concluded that the cloned gene represents aldolase B. This clone re
presents the first aldolase gene to be sequenced from any fish species
thus providing new data on the evolution of the vertebrate aldolase g
ene family.