R. Levyholtzman et I. Schechter, ACTIVITY OF THE PROMOTER OF THE HSP70 GENE OF THE PARASITIC HELMINTH SCHISTOSOMA-MANSONI IN THE MAMMALIAN CHO CELL-LINE, Biochimica et biophysica acta, N. Gene structure and expression, 1263(1), 1995, pp. 96-98
Functional assay of schistosome promoters is problematic because paras
ite cell-lines are not available. We found that the hsp70 promoters of
S. mansoni and of other eukaryotes reveal similarities in the sequenc
e and organization of the regulatory elements. Therefore, a construct
of the schistosome hsp70 promoter linked to the CAT reporter gene was
prepared and used to transfect CHO cells. The transfected cells showed
heat shock dependent activation of CAT. These findings indicate that
functional evaluation of schistosome promoters can be done, in part, i
n foster cell-lines.