EXTREME RESISTANCE TO POTATO-VIRUS-Y BASED ON THE RY-GENE IS NOT EXPRESSED IN POTATO PROTOPLASTS

Authors
HINRICHS J BERGER S BUCHENAUER H
Citation
J. Hinrichs et al., EXTREME RESISTANCE TO POTATO-VIRUS-Y BASED ON THE RY-GENE IS NOT EXPRESSED IN POTATO PROTOPLASTS, Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz, 102(3), 1995, pp. 243-248
Citations number
21
Categorie Soggetti
Plant Sciences
Journal title
Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz → ACNP
ISSN journal
03408159
Volume
102
Issue
3
Year of publication
1995
Pages
243 - 248
Database
ISI
SICI code
0340-8159(1995)102:3<243:ERTPBO>2.0.ZU;2-U
Abstract
Isolated potato protoplasts were inoculated with potato Y potyvirus (P VY) particles by polyethylene glycol (PEG). Infection of protoplasts w as determined by enzyme-linked immunosorbent assay (ELISA) using anti- PVY antibodies 1, 24 and 48 h post-inoculation (p.i.). 48 h p.i., viru s replication was supported by extreme resistant potato cultivars as e ffectively as by a susceptible cultivar. The extreme resistance to PVY of cultivars bearing the Ry-gene from Solanum stoloniferum was, there fore, not expressed by inoculated protoplasts.