PHYSICAL MAPPING AND CHARACTERIZATION OF A CATABOLIC PLASMID FROM THEDEEP-SUBSURFACE BACTERIUM SPHINGOMONAS SP STRAIN F199

Authors
STILLWELL LC THURSTON SJ SCHNEIDER RP ROMINE MF FREDRICKSON JK SAFFER JD
Citation
Lc. Stillwell et al., PHYSICAL MAPPING AND CHARACTERIZATION OF A CATABOLIC PLASMID FROM THEDEEP-SUBSURFACE BACTERIUM SPHINGOMONAS SP STRAIN F199, Journal of bacteriology, 177(15), 1995, pp. 4537-4539
Citations number
28
Categorie Soggetti
Microbiology
Journal title
Journal of bacteriology → ACNP
ISSN journal
00219193
Volume
177
Issue
15
Year of publication
1995
Pages
4537 - 4539
Database
ISI
SICI code
0021-9193(1995)177:15<4537:PMACOA>2.0.ZU;2-B
Abstract
A supercoiled 180-kb plasmid, pNL1, has been isolated from the deep-su bsurface, chemoheterotrophic Sphingomonas sp. strain F199, and a physi cal map was generated. Analysis of a pNL1-derived cosmid library indic ated that catechol 2,3-dioxygenase activity was linked to two distinct regions of the plasmid. Thus, the genes for aromatic catabolism in th is Sphingomonas strain are, at least in part, plasmid encoded.