ISOLATION OF MYOSIN LIGHT-CHAIN KINASE INHIBITORS FROM MICROORGANISMS- DEHYDROALTENUSIN, ALTENUSIN, ATROVENETINONE, AND CYCLOOCTASULFUR

Authors
NAKANISHI S TOKI S SAITOH Y TSUKUDA E KAWAHARA K ANDO K MATSUDA Y
Citation
S. Nakanishi et al., ISOLATION OF MYOSIN LIGHT-CHAIN KINASE INHIBITORS FROM MICROORGANISMS- DEHYDROALTENUSIN, ALTENUSIN, ATROVENETINONE, AND CYCLOOCTASULFUR, Bioscience, biotechnology, and biochemistry, 59(7), 1995, pp. 1333-1335
Citations number
17
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
59
Issue
7
Year of publication
1995
Pages
1333 - 1335
Database
ISI
SICI code
0916-8451(1995)59:7<1333:IOMLKI>2.0.ZU;2-N
Abstract
Dehydroaltenusin, cyclooctasulfur, atrovenetinone, and altenusin were isolated from the culture broths of Penicillium verruculosum IAM-13756 , Stretomyces verticillus subsp. tsukushiensis ATCC-21633, Penicillium sp, SPC-16375, and Penicillium sp, SPC-16524, respectively. as new my osin light chain kinase (MLCK) inhibitors. These compounds inhibited t he calmodulin-dependent activity of MLCK with IC50 values of 0.69, 0.8 6, 3.7, and 340 mu M, respectively. Among them, dehydroaltenusin was t he best MLCK inhibitor in terms of potency and selectivity examined in the purified enzyme systems.