EFFECT OF ASCORBIC-ACID SUPPLEMENTATION DURING THE INHALATION EXPOSURE OF GUINEA-PIGS TO INDUSTRIAL DUST ON BRONCHOALVEOLAR LAVAGE AND PULMONARY ENZYMES

The aim of this study was to investigate the interaction between ascor bic acid (AA) and inhaled particles separated from the dumped waste of a nickel smelter and refinery, Tricoloured male guinea pigs were expo sed in an inhalation chamber to 50 mg kg(-1) of < 5 mu m particles tha t mainly consisted of metal oxides, Exposure lasted for 4 weeks (5 day s per week and 5 h per day), The drinking water of half of the exposed and half of the control groups was supplemented with 1 g l(-1) AA. Ea ch group received 0.4 mmol kg(-1) AA in their food, Ascorbic acid supp lementation increased the pulmonary AA concentration in both exposed a nd control groups to the same extent, but in bronchoalveolar lavage fl uid the increase was higher in control than in exposed guniea pigs, Th e number of alveolar macrophages was increased by exposure and AA incr eased the number only in the exposed group; the acid phosphatase activ ity of the alveolar macrophages was increased only by AA, and more in the exposed than in the control group. Alkaline phosphatase activity i n bronchoalveolar lavage fluid was the same in both supplemented group s, but it was enhanced in the exposed group with a low intake of AA, E ffects on lactate dehydrogenase were not consistent, Neither exposure nor AA influenced significantly this enzyme in bronchoalveolar lavage fluid, but in the lung both AA and exposure caused an increase in the activity, The levels were similar in the AA-treated control and expose d guinea pigs.