HIGH-LEVEL EXPRESSION AND SIMPLE PURIFICATION OF RECOMBINANT HUMAN GRANULOCYTE-COLONY-STIMULATING FACTOR IN ESCHERICHIA-COLI

Authors
KANG SH NA KH PARK JH PARK CI LEE SY LEE YI
Citation
Sh. Kang et al., HIGH-LEVEL EXPRESSION AND SIMPLE PURIFICATION OF RECOMBINANT HUMAN GRANULOCYTE-COLONY-STIMULATING FACTOR IN ESCHERICHIA-COLI, Biotechnology letters, 17(7), 1995, pp. 687-692
Citations number
9
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
17
Issue
7
Year of publication
1995
Pages
687 - 692
Database
ISI
SICI code
0141-5492(1995)17:7<687:HEASPO>2.0.ZU;2-L
Abstract
A human granulocyte colony-stimulating factor (hG-CSF) gene was synthe sized and inserted into a trp expression vector for overexpression in E. coli. A strong expression vector was constructed, and a simple puri fication procedure including in vitro refolding was estabilished. The final productivity of hG-CSF was 500 similar to 600mg per l culture, a nd the purified hG-CSF showed the proliferation of neutrophils in vivo assays.