Je. Vermaat et Fca. Verhagen, FREEZER-INDEPENDENT PRESERVATION OF CARBOHYDRATE SAMPLES FROM WATER PLANTS - A METHODOLOGICAL TEST, Aquatic botany, 51(1-2), 1995, pp. 155-161
Two freezer-independent methods for the preservation of soluble carboh
ydrates in macrophyte tissues were tested against fresh as well as fro
zen controls, the latter jointly analysed after storage for up to 4 we
eks. Eight soluble monosaccharides and oligosaccharides were covered,
including sucrose, fructose and glucose, in photosynthetic tissues of
six species of aquatic macrophytes. Fixation in 5% formalin did not pr
ove satisfactory, while sterilisation at 110 degrees C after extractio
n did. Extraction and sterilisation can both be done by relatively sim
ple means. When reliable availability of electric power cannot be take
n for granted, e.g. in remote areas or during long-distance transport,
such methodology will prove its usefulness.