ANALYTICAL PEPTIDE-MAPPING OF A COMPLEX YELLOWFIN TUNA MYOGLOBIN PEPTIC HYDROLYSATE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

A method is described for a real identification of any peptides isolat ed from a complex peptic Yellowfin tuna red muscle myoglobin hydrolysa te. A combination of size exclusion and reversed phase high performanc e liquid chromatography have proved to be a useful strategy for fracti onation of such a mixture. This technique enable a large number of pur e peptides from the total hydrolysate to be obtained. Peptides were id entified and located on the known myoglobin sequence from their amino acid content determined by the Pico-Tag method and a second order deri vative spectroscopic method. Location of the peptides allowed us to de fine effective cut sites of the porcine pepsin. The procedure describe d in this study will be useful for acquiring a better knowledge of suc h an hydrolysate.