COMPARATIVE-STUDY ON THE ENANTIOMERIC SEPARATION OF SEVERAL NONSTEROIDAL ANTIINFLAMMATORY DRUGS ON 2 CELLULOSE-BASED CHIRAL STATIONARY PHASES

Two cellulose-based chiral stationary phases were compared on their fe asibility to resolve a representative number of 2-arylpropionic acids : a new experimental phase (Tolylcellulose, EXP B101) manufactured by Bio-Rad RSL and the Chiralcel OJ phase by Daicel. Both columns were te sted under normal phase conditions, applying a n-hexane:isopropanol mo bile phase. The Bio-Rad column was also assayed under reversed phase c onditions using a methanol and perchlorate buffer system. Enantiomeric separation without prior derivatization of most 2-arylpropionic acids was far better on the Chiralcel OJ column than on the Bio-Rad column, the latter performing somewhat better under reversed phase conditions . Prior derivatization of the carboxylic acid group with an amine (nap hthylmethylamine, (2-methyl)benzylamine) or an alcohol (benzylalcohol) permitted to separate the tested drugs to different extents on both c olumns.