IDENTIFICATION OF INDIAN WHEAT-VARIETIES BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

Biochemical techniques such as electrophoresis and reversed-phase high -performance liquid chromatography (RP-HPLC) have inherent advantages over methods based on seed morphological characteristics for cereal va rietal identification. In wheat, the major storage proteins, gliadins, are of special interest to breeders and geneticists because of their nearly invariant expression, making them accurate genotypic indicators . Similarly, protein analysis may be useful in marketing, or for seed certification. To assess the ability of one recently-developed biochem ical method to identify Indian wheats, gliadins from 13 varieties (C 3 06, WH 423, WH 416, WH 291, WH 283, WH 147, Sonalika, Kalyan Sona, HD 2189, HD 2428, HD 2329, HD 2009 and HD 2285) were analyzed by RP-HPLC. All varieties could be distinguished by comparing the resulting gliad in profiles, showing that RP-HPLC has considerable potential as an ana lytical tool to identify wheat cultivars.