URINARY ORGAN-SPECIFIC NEOANTIGEN - A POTENTIALLY DIAGNOSTIC-TEST FORCOLORECTAL-CANCER

Urinary organ-specific neoantigen from colorectal cancer patients has been used to make a monoclonal antibody, BAC 18.1. In this study we as sessed the potential of this antibody for the diagnosis of colorectal cancer. We evaluated binding in both urine and effluent samples and co mpared it with effluent carcinoembryonic antigen standardized for both volume (nanograms per milliliter) and protein. Urinary organ-specific antigen as detected by BAC 18.1 was significantly greater in 29 cance r patients (A(405): 0.717 +/- 0.500) vs 27 controls [0.121 +/- 0.273 ( P < 0.05)]. Considerable overlap of binding of BAC 18.1 was observed i n the colonic effluent of patients with CRC (N = 13), adenomas (N = 26 ), inflammatory bowel disease (N = 8), or having a normal colonoscopic examination (N = 24). CEA levels (nanograms per milliliter) were sign ificantly elevated in the effluent samples of patients with a past his tory of colorectal cancer, as compared to that of normal individuals ( P < 0.05). The presence of the Mr 30,000 organ-specific neoantigen in colonic effluent was also demonstrated by western blot. Organ-specific neoantigen originates in the colon and is excreted into the urine, so the BAC 18.1 binding levels in the urine may be a diagnostic aid for CRC.