TETHERED BENZOPHENONE REAGENTS FOR THE SYNTHESIS OF PHOTOACTIVATABLE LIGANDS

A new radiolabeled, bifunctional photoaffinity cross-linking reagent, N-succinimidyl p-benzoyl-[2,3-H-3(2)]dihydrocinnamate, has been synthe sized in high yield and with high specific activity. This reagent can be used to append the benzophenone photophore to amino groups of small molecules, such as O-aminoalkylinositol polyphosphates and polypeptid es. The resulting tritiated photoaffinity labels can be purified and m anipulated in ambient light and can be activated at 360 nm.