PHOTOIMMOBILIZATION OF A BIOACTIVE LAMININ FRAGMENT AND PATTERN-GUIDED SELECTIVE NEURONAL CELL ATTACHMENT

To attain light-dependent functionalization of biocompatible materials , a photolabel-derivatized, bioactive laminin fragment has been synthe sized, chemically characterized, and photoimmobilized. Covalent high-r esolution patterning of the laminin fragment CDPGYIGSR to hydroxylated fluorinated ethylene propylene (FEP-OH), poly(vinyl alcohol), and gly cophase glass has been achieved. The synthetic peptide CDPGYIGSR was t hermochemically coupled to either methyl)diazirin-3-yl]phenyl]-4-malei midobutyramide or 4-maleimidobenzophenone. Photolabel-derivatized pept ides were radiolabeled, and 20 and 300 mu m-sized patterns were visual ized by autoradiography. The biospecific interaction of photoimmobiliz ed laminin fragments with cells was investigated by analyzing the sele ctive attachment of NG 108-15 neuroblastoma x glioma cells which bear CDPGYIGSR-specific cell surface receptors. On photopatterned FEP-OH me mbranes NG 108-15 cells differentiated in serum-supplemented media wit hin 1 day. Specific attachment to the immobilized oligopeptide CDPGYIG SR was assessed in serum-free media with competitive binding studies, showing an 82% decrease in cell adherence after the cell receptors wer e blocked with soluble CDPGYIGSR.