ISOLATION, CHARACTERIZATION, AND PRECISE PHYSICAL LOCALIZATION OF HUMAN CDX1, A CAUDAL-TYPE HOMEOBOX GENE

The human CDX1 gene has been isolated from a small intestine cDNA Libr ary using a murine Cdx1 cDNA probe. The nucleotide sequence of CDX1 is 81% identical to murine Cdx1 and predicts a 265-amino-acid protein wi th 85% identity to the mouse protein (98% identity, including conserva tive amino acid changes). The CDX1 locus has been mapped to a cosmid c ontig from chromosome 5q31-q33, placing CDX1 approximately 100 kb dist al to CSFIR. Expression of CDX1 in adults appears to be limited to the intestine and colon by Northern analysis, suggesting a possible role in the terminal differentiation of the intestine. Further analysis of CDX1 should elucidate the function of caudal-type homeobox genes in hu man development. (C) 1995 Academic Press, Inc.