CHROMOSOMAL LOCI OF 50 HUMAN KERATINOCYTE CDNAS ASSIGNED BY FLUORESCENCE IN-SITU HYBRIDIZATION

The chromosomal loci of expressed genes provide useful information for a candidate gene approach to the genes responsible for genetic diseas es. A large set of randomly isolated cDNAs catalogued by partial seque ncing can serve as a resource for accessing and isolating these diseas e genes, Using fluorescence in situ hybridization, we examined the chr omosomal loci of 217 human keratinocyte-derived cDNAs, with independen t novel sequence tags at the 3' end region. Among them, we determined the loci of 50 cDNAs. Single-pass sequencing of these from the 5' ends indicated that 39 cDNAs still can be produced for new genes. These cD NAs with identified chromosomal loci are powerful tools that can be us ed to help elucidate the genes responsible for hereditary skin disorde rs. (C) 1995 Academic Press, Inc.