ASSIGNMENT OF THE 49-KDA (PRIM1) AND 58-KDA (PRIM2A AND PRIM2B) SUBUNIT GENES OF THE HUMAN DNA PRIMASE TO CHROMOSOME BANDS 1Q44 AND 6P11.1-P12

Authors
SHIRATORI A OKUMURA K NOGAMI M TAGUCHI H ONOZAKI T INOUE T ANDO T SHIBATA T IZUMI M MIYAZAWA H HANAOKA F MURAKAMI Y EKI T
Citation
A. Shiratori et al., ASSIGNMENT OF THE 49-KDA (PRIM1) AND 58-KDA (PRIM2A AND PRIM2B) SUBUNIT GENES OF THE HUMAN DNA PRIMASE TO CHROMOSOME BANDS 1Q44 AND 6P11.1-P12, Genomics, 28(2), 1995, pp. 350-353
Citations number
21
Categorie Soggetti
Genetics & Heredity
Journal title
Genomics → ACNP
ISSN journal
08887543
Volume
28
Issue
2
Year of publication
1995
Pages
350 - 353
Database
ISI
SICI code
0888-7543(1995)28:2<350:AOT4(A>2.0.ZU;2-Q
Abstract
DNA primase is an essential replication protein that catalyzes the syn thesis of oligoribonucleotide primers. DNA primase, consisting of two subunits (p49 and p58), plays a key role in both the initiation of DNA replication and the synthesis of Okazaki fragments for lagging strand synthesis. We mapped the locations of human chromosomes of the genes coding for both subunits [p49 (PRIM1) and p58 (PRIM2)] by PCR amplific ation using DNAs of a panel of somatic hybrids, to chromosomes 1 and 6 , respectively. The PRIM1 gene was mapped to 1q44, and two PRIM2 loci (PRIM2A and PRIM2B) were detected at 6p11.1-p12 by fluorescence in sit u hybridization using several genomic DNA probes. (C) 1995 Academic Pr ess, Inc.